Taken from a script I made a while ago for embedding lyrics into MP3 files:
http://code.activestate.com/recipes/577138-embed-lyrics-into-mp3-files-using-mutagen-uslt-tag/
The relevant part is:
from mutagen.id3 import ID3NoHeaderError
from mutagen.id3 import ID3, TIT2, TALB, TPE1, TPE2, COMM, TCOM, TCON, TDRC, TRCK
# Read the ID3 tag or create one if not present
try:
tags = ID3(fname)
except ID3NoHeaderError:
print("Adding ID3 header")
tags = ID3()
tags["TIT2"] = TIT2(encoding=3, text=title)
tags["TALB"] = TALB(encoding=3, text=u'mutagen Album Name')
tags["TPE2"] = TPE2(encoding=3, text=u'mutagen Band')
tags["COMM"] = COMM(encoding=3, lang=u'eng', desc='desc', text=u'mutagen comment')
tags["TPE1"] = TPE1(encoding=3, text=u'mutagen Artist')
tags["TCOM"] = TCOM(encoding=3, text=u'mutagen Composer')
tags["TCON"] = TCON(encoding=3, text=u'mutagen Genre')
tags["TDRC"] = TDRC(encoding=3, text=u'2010')
tags["TRCK"] = TRCK(encoding=3, text=u'track_number')
tags.save(fname)
See also: