This depends on your platform, but in general, it will perform identically.
For example, on X86, you can see this by looking at how assembly works. Check out the X86 assembly control flow operations - whether you're doing equality or inequality, it's done as 2 operations.
First, you do a CMP (comparison) operation. You then do a check to see if the comparison is equal, not equal, etc. This is just checking the results of the compare - in both cases, you're doing 2 operations.
In many higher level programming languages, however, things are different. Many languages define inequality in terms of equality - to check inequality, you do the equality check, then a second check to see if it's false. This causes equality to be (microscopically) faster in these languages. Many languages allow you to specifically write both, as well - but many people tend to write inequality in terms of equality, which again makes equality, in general, slightly faster.
==
or!=
to find that they are not equal, so the two operators are exactly equivalent. There is no reason to think one is faster than the other. – Jacobine