A cache line is a basic unit of caching. Typically it is 16-64 bytes or more.
Pentium IV: 64 bytes; Pentium Pro/II: 32 bytes; Pentium I: 32 bytes; 486: 16 bytes.
myrandomreader:
; ...
; ten instructions to generate next pseudo-random
; address in ESI from previous address
; ...
MOV EAX, DS:[ESI] ; X
LOOP myrandomreader
For memory read straddling two cachelines:
(for L1 cache miss) the processor must wait for the whole of cache line 1 to be read from L2->L1 into the processor before it can request the second cache line, causing a short execution stall
(for L2 cache miss) the processor must wait for two burst reads from L3 cache (if present) or main memory to complete rather than one
Processor stalls
A random 4 byte read will straddle a cacheline boundary about 5% of the time for 64 byte cachelines, 10% for 32 byte ones and 20% for 16 byte ones.
There may be additional execution overheads for some instructions on misaligned data even if it is within a cacheline. This is talked about on the Intel website for some SSE instructions.
If you are defining the structures yourself, it may make sense to look at listing all the <32bit data fields together in a struct
so that padding overhead is reduced or alternatively review whether it is better to turn packing on or off for a particular structure.
On MIPS and many other platforms you don't get the choice and must align - kernel exception if you don't!!
Alignment may also matter extra specially to you if you are doing I/O on the bus or using atomic operations such as atomic increment/decrement or if you wish to be able to port your code to non-Intel.
On Intel only (!) code, a common practice is to define one set of packed structures for network and disk, and another padded set for in-memory and to have routines to convert data between these formats (also consider "endianness" for the disk and network formats).