First of all, please define 'Pixel perfect' and 'Physical pixel'.
If by physical pixel you mean your display's pixel (monitor, laptop display, any other hardware you might use) then you are out of luck. Shaders don't operate on those, they operate on their own 'abstract pixels'.
You can think about it in this way:
Your graphics are rendered in a picture with some configurable resolution (say 800x600 pixels). You can still display this picture on a 1920x1080 display in full screen no problem, it would look crappy though. This is what's happening with actual display and video card rendering. What determines the actual amount of rendered pixels is your video mode (picture's resolution in the above example). And physical pixels are your display's pixels. When rendering you can only operate on the first kind.
This leads us to a conclusion that when you render the graphics at the exact same resolution as your display's native resolution, you can safely say that you endeed render it as 'Physical Pixels'.
In unity, you can pass the renderer some external data (this might include your current screen resolution (for example as a Vector2, see this).
However you most likely don't need any of this, since pixel shaders already operate on pixels (rendered pixels, determined by your current video mode). That means that if you use some resolution which is lesser than your native one, you most likely will not be able to render a single pixel.
Hope it helped.
Canvas
to be pixel-perfect. – Unassailable