AS stated in the Comment using a GroupBox Around each set of Buttons will allow each set to operate independently of each other. In the picture below I removed the GroupBox Names and lined the boarder of the groupboxes with the cell boarders then used cell boarders to almost mask the groupbox so you can't tell they are even there except for the one that is actively selected (the top most one):
On request here is some VBA code. I would suggest that before running this code so set the height of the rows to a minimum of 20 points. AS the groupbox minimum height is 19.5, Also the width of the cell will beused to determine the width of the OptionButtons, so make sure to use a width that will be roomy enough for the text of the OptionButton.
Sub Sample()
Dim Top As Variant, Left As Variant, Height As Variant, Width As Variant
Dim rngActiveRowA As Range, rngEndOfBox As Range
Dim lngActiveRow As Long, lngActiveColumn As Long
With Application
.ScreenUpdating = False
.Calculation = xlCalculationManual
.EnableEvents = False
End With
For lngActiveRow = 1 To 5
Set rngActiveRowA = Range("A" & lngActiveRow)
Set rngEndOfBox = Range("F" & lngActiveRow + 1)
Top = rngActiveRowA.Top
Left = rngActiveRowA.Left
Height = rngEndOfBox.Top - Top
Width = rngEndOfBox.Left - Left
ActiveSheet.GroupBoxes.Add(Left, Top, Width, Height).Caption = ""
For lngActiveColumn = 1 To 5
With ActiveSheet
Top = .Cells(lngActiveRow, lngActiveColumn).Top
Left = .Cells(lngActiveRow, lngActiveColumn).Left
Height = .Cells(lngActiveRow + 1, lngActiveColumn + 1).Top - Top
Width = .Cells(lngActiveRow + 1, lngActiveColumn + 1).Left - Left
End With
With ActiveSheet.OptionButtons.Add(Left, Top, Width, Height)
.Characters.Text = "OB" & lngActiveColumn
.LinkedCell = "$F$" & lngActiveRow
End With
Next lngActiveColumn
Next lngActiveRow
With Application
.ScreenUpdating = True
.Calculation = xlCalculationAutomatic
.EnableEvents = True
End With
End Sub
This code will create a groupbox around the first 5 columns of each row for the first 5 rows. It will also populate a Option Button within each cell of that area linked to the F cell in its respective row. and Rename each button to OB + the column number it is in.