You could use background-position with absolutely positioned divs as follows:
CSS:
.container {
position:relative;
height:455px;
width:606px;
}
.container div {
position:absolute;
background-image:url(http://www.beachphotos.cn/wp-content/uploads/2010/07/indoensianbeach.jpg);
}
.container .bg-image {
opacity:0.3;
height:455px;
width:606px;
}
.container div.highlight-region {
height:50px;
width:50px;
opacity:0;
}
.container div.highlight-region:hover {
opacity:1;
}
HTML:
<div class="container">
<div class="bg-image"></div>
<div class="highlight-region" style="top:50px;left:50px;background-position: -50px -50px;"></div>
<div class="highlight-region" style="top:150px;left:150px;background-position: -150px -150px;"></div>
</div>
Please see http://jsfiddle.net/MT4T7/ for an example
Credit to beachphotos.com for using their image.
EDIT (response to OP comment): Please also see http://jsfiddle.net/zLazD/ I turned off the hover aspect. also added some borders.
CSS changes:
.container div.highlight-region {
height:50px;
width:50px;
border: 3px solid white;
}
/* removed :hover section */